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pcr-script amp sk(1) cloning kit  (Agilent technologies)


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    Structured Review

    Agilent technologies pcr-script amp sk(1) cloning kit
    Pcr Script Amp Sk(1) Cloning Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr-script+sk(1)+cloning+kit/pm11096444-42-54-59
    Average 90 stars, based on 1 article reviews
    pcr-script amp sk(1) cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Increased susceptibility to Kuru of carriers of the PRNP 129 methionine/methionine genotype.
    Article Snippet: The PCR-produced fragments, representing the entire PRNP coding region, were subcloned using the pCR-Script Amp SK(1) cloning kit (Stratagene) and 7–10 subclones sequenced on an automated ABI 373A sequencer using the DyePrimer and DyeTerminator sequencing protocols (Perkin Elmer).


    Article Title: A novel mitochondrial gene order in the crinoid echinoderm Florometra serratissima.
    Article Snippet: Amplified products were either sequenced directly or cloned using one of two methods: the TA cloning method of Marchuk et al. (1990) utilizing the plasmid vector pUC19 or pBluescript II KS(1), or use of the pCR-Script Amp SK(1) cloning kit (Stratagene) according to the manufacturer’s protocol.

    Article Title: Rev-independent expression of synthetic gag-pol genes of human immunodeficiency virus type 1 and simian immunodeficiency virus: implications for the safety of lentiviral vectors.
    Article Snippet: The safety of lentiviral vectors for clinical applications is still a major concern.. The gag–pol expression plasmids and the lentiviral vectors used in previous studies contain homologous regions, which constitute a risk for recombination events.. Synthetic gag–pol genes of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) were therefore constructed, in which the codon usage was optimized for expression in human cells without altering the amino acid sequences.

    Article Title: DNA damaging agents increase gadd153 (CHOP-10) messenger RNA levels in bovine preimplantation embryos cultured in vitro.
    Article Snippet: The PCR product was purified from a 1% agarose gel and cloned into the SrfI site of the pCR-Script SK(1) plasmid using the pCR-Script Amp SK(1) Cloning Kit (Stratagene, Cambridge, UK).

    Article Title: Recombination among multiple mitochondrial pseudogenes from a Passerine genus.
    Article Snippet: 2 f tein coding subunit 5 of NADH-dehydrogenase (ND5) from eight individuals representing five species of the South American bird genus Conirostrum were cloned.. The 130 clones, which were subsequently sequenced, constituted 55 different sequences.. Due to the observed differences in substitution patterns 58% of the cloned sequences were identified as pseudogenes.

    Polymerase Chain Reaction:

    Article Title: Reassessment of homology of morphological characters in tetractinellid sponges based on molecular data.
    Article Snippet: .. The ® rst cycle is 4 min at 94 8 C, 2 min at Ta, and 2 min at 72 8 C followed by 30 cycles each consisting of 1 min at 94 8 C, 1 min at Ta, and 1 min at 72 8 C. The reaction is ® nished by 4 min at 72 8 C. Each PCR fragment was cloned into PCRScript SK(1 ) Cloning Vector (PCR-Script SK(1 ) Cloning Kit, Stratagene) and sequenced with the T7 Sequencing Kit (Pharmacia Biotech) using [33P]dATP and DMSO in the annealing reaction. ..

    Clone Assay:

    Article Title: Reassessment of homology of morphological characters in tetractinellid sponges based on molecular data.
    Article Snippet: .. The ® rst cycle is 4 min at 94 8 C, 2 min at Ta, and 2 min at 72 8 C followed by 30 cycles each consisting of 1 min at 94 8 C, 1 min at Ta, and 1 min at 72 8 C. The reaction is ® nished by 4 min at 72 8 C. Each PCR fragment was cloned into PCRScript SK(1 ) Cloning Vector (PCR-Script SK(1 ) Cloning Kit, Stratagene) and sequenced with the T7 Sequencing Kit (Pharmacia Biotech) using [33P]dATP and DMSO in the annealing reaction. ..

    Cloning:

    Article Title: Reassessment of homology of morphological characters in tetractinellid sponges based on molecular data.
    Article Snippet: .. The ® rst cycle is 4 min at 94 8 C, 2 min at Ta, and 2 min at 72 8 C followed by 30 cycles each consisting of 1 min at 94 8 C, 1 min at Ta, and 1 min at 72 8 C. The reaction is ® nished by 4 min at 72 8 C. Each PCR fragment was cloned into PCRScript SK(1 ) Cloning Vector (PCR-Script SK(1 ) Cloning Kit, Stratagene) and sequenced with the T7 Sequencing Kit (Pharmacia Biotech) using [33P]dATP and DMSO in the annealing reaction. ..

    Sequencing:

    Article Title: Reassessment of homology of morphological characters in tetractinellid sponges based on molecular data.
    Article Snippet: .. The ® rst cycle is 4 min at 94 8 C, 2 min at Ta, and 2 min at 72 8 C followed by 30 cycles each consisting of 1 min at 94 8 C, 1 min at Ta, and 1 min at 72 8 C. The reaction is ® nished by 4 min at 72 8 C. Each PCR fragment was cloned into PCRScript SK(1 ) Cloning Vector (PCR-Script SK(1 ) Cloning Kit, Stratagene) and sequenced with the T7 Sequencing Kit (Pharmacia Biotech) using [33P]dATP and DMSO in the annealing reaction. ..



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